Myrsini Margariti was born in Larissa/Greece. She studied violin and singing at the National Conservatory in Athens and Musicology at the University of Athens. She continued her education with Prof. Elisabeth Wilke and Prof. Gudrun Volkert at the University Mozarteum in Salzburg. She completed two Master’s degrees in “Lied and Oratorio” (Class Prof. H. Höll and B. Zakotnik) «Opera and Musical Theater» (Class Prof. W. Kolneder and R. Seifreid) with the highest honors. She was granted with the “Alexander Onassis Foundation” and the “Juergen Ponto Stiftung” Scholarship. During her studies she also attended master classes given by A. Csengery, A. West, F. Woutsinos, A. Markert, H. Rilling, G. Pisarenko, G. Janowitz, W. Holzmair, Chr. Rousset.
During her studies she already performed the roles of Gretel (Hänsel & Gretel, Salzburg), Muzio (World premiere of the new – discovered baroque Opera of Cesarini, Scarlatti and Caldara: Giunio Bruto, tournee in Austria and Germany), Little red riding hood (Little red riding hood and the good Wolf, Athens), Zerlina (Don Giovanni – “Mozart's Year”, Salzburg), Serpetta (La finta giardiniera – Landestheater Salzburg in cooperation with the International Salzburg Festival).
From 2006 to 2009 Myrsini Margariti has been a member of the ensemble at the Opera of Halle in Germany, the hometown of Handel, where she has performed several roles with great success. She has been seen and heard as:
Pamina (Dia Zauberflöte), Gretel (Hänsel und Gretel), Zerlina (Don Giovanni), Euridice (L' Orfeo), Wellgunde (Das Rheingold), Xenia (Boris Godunov), ( Suor Genovieffa in (Suor Angelica), Zerlina (Don Giovanni), Tebaldo and Voce dal cielo (Don Carlo), Sopranistin (Hochzeiten und andere Katastrophen), Venus (Frau Luna) a.o.
She made guest appearances at the Theater of Erfurt, Staatstheater Darmstadt, Theater of Fuerth, International Haendel Festspiele Halle, Opera Halle, Theater Wintertur (Swiss), Opera Festival of ancient Corinth, Opera of Larissa, Megaron Athens ,la piccola Scala in Syros (Festival of the aegean),Benaki Museum Athens , Athens Festival in roles such as Pamina, Micaela, Euridice, Violetta, Drusilla a.o. In September 2010 she made a tournee in Mexico with the Westsaechsische Symphonieorchester.

Her repertoire includes numerous roles in the genres of Opera, Operetta and Musical Theater, as well as several Lied cycles and a wide variety of concert literature, while her performances range from the baroque to the contemporary. As a concert singer Myrsini has performed extensively in Greece, Austria, Germany, Hungary.
She has collaborated with conductors such as Helmuth Rilling (Bachakademie), Marcus Creed, George Petrou, Karl-Heinz Steffens a.o.
Her interpretation in the recording of Handel's Cantata Apollo e Dafne (Händelfestspielorchester Halle, Bernhard Forck, Production: AVI -Music) received critical acclaim.